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使用形态基因的农业细菌 -顽固玉米近亲繁殖系的介导未成熟胚胎转化

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

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使用形态基因的农业细菌 -顽固玉米近亲繁殖系的介导未成熟胚胎转化

DOI:

10.3791/60782-v

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10:28 min

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February 14, 2020

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Alicia Masters², Minjeong Kang^3,4, Morgan McCaw³, Jacob D. Zobrist^3,5, William Gordon-Kamm, Todd Jones, Kan Wang³

¹Department of Agronomy,Iowa State University, ²Department of Applied Science and Technology,Corteva Agriscience, ³Crop Bioengineering Center,Iowa State University, ⁴Interdepartmental Plant Biology Major,Iowa State University, ⁵Interdepartmental Genetics and Genomics Major,Iowa State University

Capítulos

00:05Introduction
01:01Growing Donor Plants and Harvesting Immature Ears
02:40Embryo Dissection, Infection, and Co-cultivation
05:54Selection, Heat Treatment, and Regeneration
07:32Greenhouse Transplantation and T1 Seed Production
08:08Results: Representative Agrobacterium-mediated Immature Embryo Transformation
09:27Conclusion

Summary

Tadução automática

植物形态基因可用于改善顽固基因型的基因转化。这里描述的是三个重要的公共玉米近亲繁殖线的农业细菌介导遗传转化（QuickCorn）协议。

Tags

Keywords: Agrobacterium-mediated Immature Embryo Transformation Recalcitrant Maize Inbred Lines Morphogenic Genes BABY BOOM WUSCHEL QuickCorn Callus Induction T-DNA Cre/loxP Heat-induced Recombination Embryogenic Growth Pollen Pollination Immature Embryo Size Embryo Extraction

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