Journal
/
Biologia
/
Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

21,074 Views

•

10:28 min

•

February 14, 2020

DOI:

10.3791/60782-v

DOI:
10.3791/60782-v

•

10:28 min
February 14, 2020

•

15 Views
, , , , , ,
1Department of Agronomy,Iowa State University, 2Department of Applied Science and Technology,Corteva Agriscience, 3Crop Bioengineering Center,Iowa State University, 4Interdepartmental Plant Biology Major,Iowa State University, 5Interdepartmental Genetics and Genomics Major,Iowa State University

Explore More Videos

Agrobacterium-mediatedImmature EmbryoTransformationRecalcitrantMaizeInbred LinesMorphogenic GenesBABY BOOMWUSCHELQuickCornCallus InductionT-DNACre/loxPHeat-induced RecombinationEmbryogenic GrowthPollenPollinationImmature Embryo SizeEmbryo Extraction

Read Article

Cite this Article

Masters, A., Kang, M., McCaw, M., Zobrist, J. D., Gordon-Kamm, W., Jones, T., Wang, K. Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes. J. Vis. Exp. (156), e60782, doi:10.3791/60782 (2020).

Download .ris file

Copy

Share Video

.

Copy