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小鼠胚胎细胞的多路复用单细胞mRNA测序分析
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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小鼠胚胎细胞的多路复用单细胞mRNA测序分析

DOI:
10.3791/60647-v

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08:30 min

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January 07, 2020

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, ,
1Department of Developmental Biology,University of Pittsburgh School of Medicine

Capítulos

  • 00:04Título
  • 00:41Lipid-based Barcoding
  • 01:36Droplet Generation and mRNA Reverse Transcription
  • 03:43Post Single Cell Reverse Transcription Cleanup
  • 05:18cDNA Amplification
  • 06:42Results: Representative Multiplexed Single Cell mRNA Sequencing Analysis
  • 07:50Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们提出了一种多路复用单细胞mRNA测序方法，用于分析小鼠胚胎组织中的基因表达。基于滴滴的单细胞mRNA测序（scRNA-Seq）方法与多路复用策略相结合，可以同时分析来自多个样品的单个细胞，从而显著降低试剂成本并最大限度地减少实验批次效应。

Tags

Single Cell MRNA SequencingMultiplexingCell IsolationCell PreparationCell CountingAnchor BarcodeCo-anchor BarcodeCell FilteringChip AssemblyCell Solution PreparationGel Bead AdditionPartitioning OilChromium ControllerSingle Cell Analysis

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