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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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Biologie du développement
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Journal JoVE Biologie du développement
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

DOI:
10.3791/60647-v

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08:30 min

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January 07, 2020

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, ,
1Department of Developmental Biology,University of Pittsburgh School of Medicine

Chapitres

  • 00:04Titre
  • 00:41Lipid-based Barcoding
  • 01:36Droplet Generation and mRNA Reverse Transcription
  • 03:43Post Single Cell Reverse Transcription Cleanup
  • 05:18cDNA Amplification
  • 06:42Results: Representative Multiplexed Single Cell mRNA Sequencing Analysis
  • 07:50Conclusion

Summary

Traduction automatique

Traduction automatique

Here we presented a multiplexed single cell mRNA sequencing method to profile gene expression in mouse embryonic tissues. The droplet-based single cell mRNA sequencing (scRNA-Seq) method in combination with multiplexing strategies can profile single cells from multiple samples simultaneously, which significantly reduces reagent costs and minimizes experimental batch effects.

Tags

Keywords: Single Cell MRNA SequencingMultiplexingCell IsolationCell PreparationCell CountingAnchor BarcodeCo-anchor BarcodeCell FilteringChip AssemblyCell Solution PreparationGel Bead AdditionPartitioning OilChromium ControllerSingle Cell Analysis

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