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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

12,244 Views

08:30 min

January 07, 2020

DOI:

08:30 min
January 07, 2020

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Summary

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Here we presented a multiplexed single cell mRNA sequencing method to profile gene expression in mouse embryonic tissues. The droplet-based single cell mRNA sequencing (scRNA-Seq) method in combination with multiplexing strategies can profile single cells from multiple samples simultaneously, which significantly reduces reagent costs and minimizes experimental batch effects.

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