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Developmental Biology

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JoVE Journal Developmental Biology
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
 

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Article DOI: 10.3791/60647-v • 08:30 min • January 7th, 2020
January 7th, 2020 •
1, 1, 1
1Department of Developmental Biology, University of Pittsburgh School of Medicine

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Here we presented a multiplexed single cell mRNA sequencing method to profile gene expression in mouse embryonic tissues. The droplet-based single cell mRNA sequencing (scRNA-Seq) method in combination with multiplexing strategies can profile single cells from multiple samples simultaneously, which significantly reduces reagent costs and minimizes experimental batch effects.

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