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Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

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06:53 min

October 15, 2019

DOI:

06:53 min
October 15, 2019

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Summary

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Myoblasts are proliferating precursor cells that differentiate to form polynucleated myotubes and eventually skeletal muscle myofibers. Here, we present a protocol for efficient isolation and culture of primary myoblasts from young adult mouse skeletal muscles. The method enables molecular, genetic, and metabolic studies of muscle cells in culture.

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