Myoblasts are proliferating precursor cells that differentiate to form polynucleated myotubes and eventually skeletal muscle myofibers. Here, we present a protocol for efficient isolation and culture of primary myoblasts from young adult mouse skeletal muscles. The method enables molecular, genetic, and metabolic studies of muscle cells in culture.
Vaughan, M., Lamia, K. A. Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants. J. Vis. Exp. (152), e60310, doi:10.3791/60310 (2019).