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Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
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Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
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October 15, 2019
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Myoblasts are proliferating precursor cells that differentiate to form polynucleated myotubes and eventually skeletal muscle myofibers. Here, we present a protocol for efficient isolation and culture of primary myoblasts from young adult mouse skeletal muscles. The method enables molecular, genetic, and metabolic studies of muscle cells in culture.
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Vaughan, M., Lamia, K. A. Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants. J. Vis. Exp. (152), e60310, doi:10.3791/60310 (2019).
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