ジャーナル
/
/
Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

16,074 Views

06:53 min

October 15, 2019

DOI:

06:53 min
October 15, 2019

31 Views
,

概要

Automatically generated

Myoblasts are proliferating precursor cells that differentiate to form polynucleated myotubes and eventually skeletal muscle myofibers. Here, we present a protocol for efficient isolation and culture of primary myoblasts from young adult mouse skeletal muscles. The method enables molecular, genetic, and metabolic studies of muscle cells in culture.

Read Article