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使用流感抗原微阵列测量病毒亚型血清抗体的广度
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
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使用流感抗原微阵列测量病毒亚型血清抗体的广度

DOI:
10.3791/59973-v

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08:52 min

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July 26, 2019

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, , , , , , , , , , , , , , ,
1Division of Infectious Diseases, Department of Medicine,University of California Irvine Health, 2Vaccine Research and Development Center, Department of Physiology,University of California Irvine

Capítulos

  • 00:04Título
  • 01:27Antigen Microarray Printing
  • 03:01Probe Sera Microarray Incubation
  • 04:25Quantum-Dot-Conjugated Secondary Antibody Labeling
  • 05:16Antibody Binding Capture
  • 06:03Antibody Binding Quantification
  • 07:00Results: Representative Influenza Antigen Microarray Analysis
  • 08:03Conclusion

Summary

Tadução automática

Tadução automática

我们提出了一种使用通过将流感抗原打印到硝基纤维素涂层幻灯片上构建的蛋白质微阵列的协议,以同时针对来自不同病毒株的 250 多种抗原检测血清的多种抗体等型,从而允许测量病毒亚型血清抗体的广度。

Tags

Influenza Antigen MicroarrayAntibody BreadthVirus SubtypesHigh-throughput MeasurementAntibody CharacterizationMicroarray PrintingAntigen PrintingMicroarray Probing

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