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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
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JoVE Journal Genética
Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

9,040 Views

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11:30 min

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March 24, 2020

DOI:

10.3791/59955-v

DOI:
10.3791/59955-v

•

11:30 min
March 24, 2020

•

4 Views
, , ,
1Toxicology Program, ILS, Inc., 2Division of the National Toxicology Program,National Institute of Environmental Health Sciences

Summary

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This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.

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Hobbs, C. A., Recio, L., Winters, J., Witt, K. L. Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage. J. Vis. Exp. (157), e59955, doi:10.3791/59955 (2020).

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