このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
9,040 Views
•
11:30 min
•
March 24, 2020
DOI:
概要
Automatically generated
This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.
Read Article
Cite this Article
Hobbs, C. A., Recio, L., Winters, J., Witt, K. L. Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage. J. Vis. Exp. (157), e59955, doi:10.3791/59955 (2020).
Copy