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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

9,040 Views

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11:30 min

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March 24, 2020

DOI:

10.3791/59955-v

DOI:
10.3791/59955-v

•

11:30 min
March 24, 2020

•

4 Views
, , ,
1Toxicology Program, ILS, Inc., 2Division of the National Toxicology Program,National Institute of Environmental Health Sciences

Summary

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This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.

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Hobbs, C. A., Recio, L., Winters, J., Witt, K. L. Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage. J. Vis. Exp. (157), e59955, doi:10.3791/59955 (2020).

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