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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
Capítulos
- 00:04Título
- 00:50Patterned Cell Culture Light Microscopy
- 02:18Electron Microscopy (EM) Sample Block Preparation
- 04:46Scanning Electron Microscopy (SEM) Section Preparation
- 06:40Resultados
- 08:20Conclusion
We present a protocol to study the distribution of mitochondria and endoplasmic reticulum in whole cells after genetic modification using correlative light and volume electron microscopy including ascorbate peroxidase 2 and horseradish peroxidase staining, serial sectioning of cells with and without the target gene in the same section, and serial imaging via electron microscopy.
