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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
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Biochemistry
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JoVE Journal Biochemistry
Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

DOI:
10.3791/59750-v

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09:21 min

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July 20, 2019

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,
1Neural Circuits Research Group,Korea Brain Research Institute

Chapters

  • 00:04Title
  • 00:50Patterned Cell Culture Light Microscopy
  • 02:18Electron Microscopy (EM) Sample Block Preparation
  • 04:46Scanning Electron Microscopy (SEM) Section Preparation
  • 06:40Results
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to study the distribution of mitochondria and endoplasmic reticulum in whole cells after genetic modification using correlative light and volume electron microscopy including ascorbate peroxidase 2 and horseradish peroxidase staining, serial sectioning of cells with and without the target gene in the same section, and serial imaging via electron microscopy.

Tags

Keywords: MitochondriaEndoplasmic ReticulumCorrelative Light MicroscopyVolume Electron Microscopy3-D StructureElectron MicroscopeDAB StainingOsmium TetroxideTCH SolutionMembranous OrganellesHost Cells

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