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Biochimie
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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
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Biochimie
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Journal JoVE Biochimie
Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

DOI:
10.3791/59750-v

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09:21 min

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July 20, 2019

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,
1Neural Circuits Research Group,Korea Brain Research Institute

Chapitres

  • 00:04Titre
  • 00:50Patterned Cell Culture Light Microscopy
  • 02:18Electron Microscopy (EM) Sample Block Preparation
  • 04:46Scanning Electron Microscopy (SEM) Section Preparation
  • 06:40Résultats
  • 08:20Conclusion

Summary

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Traduction automatique

We present a protocol to study the distribution of mitochondria and endoplasmic reticulum in whole cells after genetic modification using correlative light and volume electron microscopy including ascorbate peroxidase 2 and horseradish peroxidase staining, serial sectioning of cells with and without the target gene in the same section, and serial imaging via electron microscopy.

Tags

MitochondriaEndoplasmic ReticulumCorrelative Light MicroscopyVolume Electron Microscopy3-D StructureElectron MicroscopeDAB StainingOsmium TetroxideTCH SolutionMembranous OrganellesHost Cells

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