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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
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July 20, 2019
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We present a protocol to study the distribution of mitochondria and endoplasmic reticulum in whole cells after genetic modification using correlative light and volume electron microscopy including ascorbate peroxidase 2 and horseradish peroxidase staining, serial sectioning of cells with and without the target gene in the same section, and serial imaging via electron microscopy.
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Jung, M., Mun, J. Y. Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy. J. Vis. Exp. (149), e59750, doi:10.3791/59750 (2019).
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