Journal
/
Neurociência
/
An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

DOI:
10.3791/59433-v

•

05:51 min

•

May 23, 2019

•

, , ,
1Department of Neurosciences,University of California, San Diego

Capítulos

  • 00:04Título
  • 00:27Preparation of Media and Reagents
  • 01:41Culturing Human Neural Stem Cells
  • 03:12Transduction and Maintenance of Neuronal Cultures
  • 04:05Live-cell Imaging
  • 04:32Results: Analysis of Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
  • 05:22Conclusion

Summary

Tadução automática

Tadução automática

This protocol details a procedure in which human neuronal cultures are transduced with lentiviral constructs coding for mutant human tau. Transduced cultures display tau aggregates and associated pathologies.

Tags

Tau AggregationIn Vitro ModelHuman NeuronsLentiviral TransductionCell CultureDrug ScreeningDisease MechanismBasement Membrane MatrixNeural Stem Cell MediaBFGFDMEM-F12Penicillin-streptomycinCell ThawingCentrifugationCell SeedingConfluencyLentivirus Transduction

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

Studying the Integration of Adult-born Neurons

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

An In Vitro Model for the Study of Cellular Pathophysiology in Globoid Cell Leukodystrophy

An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

Read Article