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An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
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JoVE Journal Neurowissenschaften
An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

5,710 Views

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05:51 min

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May 23, 2019

DOI:

10.3791/59433-v

DOI:
10.3791/59433-v

•

05:51 min
May 23, 2019

•

20 Views
, , ,
1Department of Neurosciences,University of California, San Diego

Summary

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This protocol details a procedure in which human neuronal cultures are transduced with lentiviral constructs coding for mutant human tau. Transduced cultures display tau aggregates and associated pathologies.

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Tau AggregationIn Vitro ModelHuman NeuronsLentiviral TransductionCell CultureDrug ScreeningDisease MechanismBasement Membrane MatrixNeural Stem Cell MediaBFGFDMEM-F12Penicillin-streptomycinCell ThawingCentrifugationCell SeedingConfluencyLentivirus Transduction

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Aulston, B., Liu, Q., Reilly, P., Yuan, S. H. An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons. J. Vis. Exp. (147), e59433, doi:10.3791/59433 (2019).

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