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Biologia do Desenvolvimento

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全山荧光在小鼠胚胎发生过程中心脏室发育的分析

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

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全山荧光在小鼠胚胎发生过程中心脏室发育的分析

DOI:

10.3791/59413-v

•

06:27 min

•

April 17, 2019

•

Zhentao Zhang^2,3, Young-Jae Nam^2,3

¹Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University Medical Center, ²Department of Cell and Developmental Biology,Vanderbilt University, ³Vanderbilt Center for Stem Cell Biology,Vanderbilt University

Capítulos

00:04Título
00:45Mouse Embryo Collection and Dissection
02:39Whole-mount Epifluorescence Imaging
03:23Genotyping
04:34Results: Cardiac Chamber Development During Mouse Embryogenesis in MLC-2v-tdTomato Knock-in Mice
06:02Conclusion

Summary

Tadução automática

我们提出的方案, 以检查小鼠心脏的发展使用整个安装视网膜显微镜从心室特异性 Mlc-2v-t分解番茄记者敲入小鼠解剖小鼠胚胎。这种方法使我们能够直接想象小鼠心脏发育过程中心室形成的每个阶段, 而无需劳动密集型组织化学方法。

Tags

Keywords: Cardiac Chamber Development Mouse Embryogenesis Whole Mount Epifluorescence MLC-2v-tdTomato Heart Tube Formation Looping Chamber Formation Fluorescent Reporter Dissection Genotyping Epifluorescent Imaging

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