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Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

DOI:
10.3791/58592-v

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11:21 min

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November 20, 2018

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, ,
1Department of Pharmacology,University College London

Capítulos

  • 00:04Título
  • 00:26Tissue Preparation and Staining for Fluorescence Microscopy and 3D Neuronal Reconstruction
  • 04:08Histological Detection of Biotin-filled Neurons
  • 08:033D Neuronal Reconstructions
  • 10:12Results: Reconstruction of a Basket Cell Recorded and Filled in the Hippocampal CA2 Region and Morphometry Analyses
  • 10:57Conclusion

Summary

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The protocol outlined here describes the immunofluorescence analysis, biocytin recovery and high-quality reconstructions of hippocampal CA2 interneurons following the intracellular electrophysiological recordings in vitro, allowing neuronal characterization and ultimately fine neuronal anatomy to be studied.

Tags

Biocytin3D ReconstructionHippocampal CA2 InterneuronsNeuronal SubtypesCortical CircuitryUltrastructureDendritic ArborAxonal ArborElectrophysiologyACSFFixative SolutionPhosphate BufferGelatinVibratomeSucrose

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