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Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

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11:21 min

November 20, 2018

DOI:

11:21 min
November 20, 2018

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Summary

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The protocol outlined here describes the immunofluorescence analysis, biocytin recovery and high-quality reconstructions of hippocampal CA2 interneurons following the intracellular electrophysiological recordings in vitro, allowing neuronal characterization and ultimately fine neuronal anatomy to be studied.

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