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Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
章
- 00:04標題
- 00:26Tissue Preparation and Staining for Fluorescence Microscopy and 3D Neuronal Reconstruction
- 04:08Histological Detection of Biotin-filled Neurons
- 08:033D Neuronal Reconstructions
- 10:12Results: Reconstruction of a Basket Cell Recorded and Filled in the Hippocampal CA2 Region and Morphometry Analyses
- 10:57Conclusion
The protocol outlined here describes the immunofluorescence analysis, biocytin recovery and high-quality reconstructions of hippocampal CA2 interneurons following the intracellular electrophysiological recordings in vitro, allowing neuronal characterization and ultimately fine neuronal anatomy to be studied.
