The protocol outlined here describes the immunofluorescence analysis, biocytin recovery and high-quality reconstructions of hippocampal CA2 interneurons following the intracellular electrophysiological recordings in vitro, allowing neuronal characterization and ultimately fine neuronal anatomy to be studied.
Economides, G., Falk, S., Mercer, A. Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons. J. Vis. Exp. (141), e58592, doi:10.3791/58592 (2018).