Journal
/
Bioquímica
/
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

6,788 Views

•

07:46 min

•

October 08, 2018

DOI:

10.3791/58100-v

DOI:
10.3791/58100-v

•

07:46 min
October 08, 2018

•

1 Views
, , , , ,
1Department of Biology,Ball State University, 2Department of Genome Sciences,University of Washington, 3Department of Molecular, Cellular, and Developmental Biology,University of Michigan

Explore More Videos

TRNA-Isopentenyl TransferaseIn Vitro AssayRNA ModificationBiochemical CharacterizationRecombinant EnzymesRNA Modifying EnzymesAcrylamide GelUrea GelTBE BufferRNA Isopentenylation AssayDMAPPMod52-mercaptoethanolRNA Precipitation

Read Article

Cite this Article

Chambers, A. E., Richardson, A. E., Read, D. F., Waller, T. J., Bernstein, D. A., Smaldino, P. J. An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity. J. Vis. Exp. (140), e58100, doi:10.3791/58100 (2018).

Download .ris file

Copy

Share Video

.

Copy