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使用层粘连蛋白521矩阵整合人诱导多能干细胞的自由衍生

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

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使用层粘连蛋白521矩阵整合人诱导多能干细胞的自由衍生

DOI:

10.3791/56146-v

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10:48 min

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July 07, 2017

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Elias Uhlin, Ana Marin Navarro², Harriet Rönnholm, Kelly Day, Malin Kele, Anna Falk

¹Department of Neuroscience,Karolinska Institutet, ²Department of Microbiology, Tumor and Cell Biology,Karolinska Institutet

Capítulos

00:05Título
01:08Biopsy Handling
03:42Sendai Virus Vector Reprogramming of Fibroblasts
05:17Picking of Colonies and Expansion of hiPS Cells
08:21Results: Successful Generation of Defined and Xeno-free hiPS Cells from Fiboblasts
10:10Conclusion

Summary

Tadução automática

通过使用非整合仙台病毒（SeV）载体介导的真皮成纤维细胞的重编程，实现人诱导的多能干细胞（hiPS）细胞的稳健衍生。使用重组人层粘连蛋白521（LN-521）基质和Essential E8（E8）培养基，使用无异种素和化学定义的培养条件进行hiPS细胞维持和克隆扩增。

Tags

Keywords: Human Induced Pluripotent Stem Cells Integration-free Derivation Laminin 521 Matrix Skin Biopsy Dispase Collagenase I Xeno-free Chemically Defined Culture System Disease Modeling Drug Screening Cell Therapy

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