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Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

DOI:
10.3791/56146-v

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10:48 min

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July 07, 2017

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, , , , ,
1Department of Neuroscience,Karolinska Institutet, 2Department of Microbiology, Tumor and Cell Biology,Karolinska Institutet

Kapitel

  • 00:05Titel
  • 01:08Biopsy Handling
  • 03:42Sendai Virus Vector Reprogramming of Fibroblasts
  • 05:17Picking of Colonies and Expansion of hiPS Cells
  • 08:21Results: Successful Generation of Defined and Xeno-free hiPS Cells from Fiboblasts
  • 10:10Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.

Tags

Human Induced Pluripotent Stem CellsIntegration-free DerivationLaminin 521 MatrixSkin BiopsyDispaseCollagenase IXeno-freeChemically Defined Culture SystemDisease ModelingDrug ScreeningCell Therapy

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