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Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
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发育生物学
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JoVE 杂志 发育生物学
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

8,381 Views

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10:48 min

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July 07, 2017

DOI:

10.3791/56146-v

DOI:
10.3791/56146-v

•

10:48 min
July 07, 2017

•

2 Views
, , , , ,
1Department of Neuroscience,Karolinska Institutet, 2Department of Microbiology, Tumor and Cell Biology,Karolinska Institutet

Summary

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Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.

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Cite this Article

Uhlin, E., Marin Navarro, A., Rönnholm, H., Day, K., Kele, M., Falk, A. Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix. J. Vis. Exp. (125), e56146, doi:10.3791/56146 (2017).

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