/
/
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
8,382 Views
•
10:48 min
•
July 07, 2017
DOI:
Summary
Automatically generated
Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.
Read Article
Cite this Article
Uhlin, E., Marin Navarro, A., Rönnholm, H., Day, K., Kele, M., Falk, A. Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix. J. Vis. Exp. (125), e56146, doi:10.3791/56146 (2017).
Copy