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Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

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10:48 min

July 07, 2017

DOI:

10:48 min
July 07, 2017

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Summary

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Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.

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