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EPA Method 1615. Måling av Enterovirus og Norovirus Forekomst i vann etter kultur og RT-qPCR. II. Total dyrkbare Virus analysen
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Ambiente
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JoVE Journal Ambiente
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay

EPA Method 1615. Måling av Enterovirus og Norovirus Forekomst i vann etter kultur og RT-qPCR. II. Total dyrkbare Virus analysen

8,398 Views

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11:09 min

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September 11, 2016

DOI:

10.3791/52437-v

DOI:
10.3791/52437-v

•

11:09 min
September 11, 2016

•

11 Views
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1National Exposure Research Laboratory,U.S. Environmental Protection Agency

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EPA Method 1615Virus MeasurementTotal Culturable Virus AssayAlkaline-based Extract SolutionElectro-positive FilterVirus ElutionVirus ConcentrationVirus FlocculationVirus IsolationBuffalo Green Monkey Kidney CellsCytopathic Effect (CPE)Most Probable Number (MPN)EnterovirusNorovirusDrinking WaterEnvironmental WaterWater QualityVirus DetectionVirus Quantification

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Fout, G. S., Cashdollar, J. L. EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay. J. Vis. Exp. (115), e52437, doi:10.3791/52437 (2016).

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