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強化された骨形成のためのヒト脂肪由来間質細胞の単離および濃縮
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
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強化された骨形成のためのヒト脂肪由来間質細胞の単離および濃縮

11,295 Views

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11:00 min

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January 12, 2015

DOI:

10.3791/52181-v

DOI:
10.3791/52181-v

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11:00 min
January 12, 2015

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3 Views
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1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University

Summary

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The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.

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Zielins, E. R., Tevlin, R., Hu, M. S., Chung, M. T., McArdle, A., Paik, K. J., Atashroo, D., Duldulao, C. R., Luan, A., Senarath-Yapa, K., Walmsley, G. G., Wearda, T., Longaker, M. T., Wan, D. C. Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis. J. Vis. Exp. (95), e52181, doi:10.3791/52181 (2015).

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