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Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

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11:00 min

January 12, 2015

DOI:

11:00 min
January 12, 2015

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Özet

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The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.

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