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JoVE Journal Neuroscience
Langsigtet Time Lapse Imaging af Mouse Cochlear Eksplantater
 
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Langsigtet Time Lapse Imaging af Mouse Cochlear Eksplantater

Article DOI: 10.3791/52101-v • 10:43 min • November 2nd, 2014
November 2nd, 2014 •
1, 1,2,3
1Biological Sciences Platform, Sunnybrook Research Institute, 2Department of Otolaryngology - Head and Neck Surgery, University of Toronto, 3Department of Laboratory Medicine and Pathobiology, University of Toronto

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Live-billeddannelse af embryonale pattedyr cochlea er udfordrende, fordi de udviklingsmæssige processer ved hånden operere på en tidsmæssig gradient i løbet af ti dage. Her præsenteres en fremgangsmåde til dyrkning og derefter afbilde embryonale cochlear eksplantat væv taget fra en fluorescerende reporter musen over fem dage.

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Bioengineering live-billeddannelse tid lapse cochlea øre reporter mus udvikling inkubator mikroskop Sox2
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