Journal
/
Neurociência
/
Long-term Time Lapse Imaging of Mouse Cochlear Explants
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Long-term Time Lapse Imaging of Mouse Cochlear Explants

Long-term Time Lapse Imaging of Mouse Cochlear Explants

DOI:
10.3791/52101-v

•

10:43 min

•

November 02, 2014

•

,
1Biological Sciences Platform,Sunnybrook Research Institute, 2Department of Otolaryngology – Head and Neck Surgery,University of Toronto, 3Department of Laboratory Medicine and Pathobiology,University of Toronto

Capítulos

  • 00:05Título
  • 01:31Culturing Embryonic Cochleae and Explants
  • 05:56Live Imaging
  • 08:57Results: Cochlear Explant Time Lapse
  • 10:06Conclusion

Summary

Tadução automática

Tadução automática

Live imaging of the embryonic mammalian cochlea is challenging because the developmental processes at hand operate on a temporal gradient over ten days. Here we present a method for culturing and then imaging embryonic cochlear explant tissue taken from a fluorescent reporter mouse over five days.

Tags

Keywords: Cochlear ExplantTime-lapse ImagingLive ImagingEmbryonic MouseMammalian CochleaGene ExpressionFluorescent MicroscopyBright Field MicroscopyIncubator MicroscopeSox2Prosensory Cells

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/52101?article_type=v

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
check_url/pt/52101?article_type=v

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain
check_url/pt/52101?article_type=v

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain
check_url/pt/52101?article_type=v

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain
check_url/pt/52101?article_type=v

Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish
check_url/pt/52101?article_type=v

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
check_url/pt/52101?article_type=v

Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans
check_url/pt/52101?article_type=v

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
check_url/pt/52101?article_type=v

Generation and Long-term Maintenance of Nerve-free Hydra
check_url/pt/52101?article_type=v

Long-term Sensory Conflict in Freely Behaving Mice

Read Article