Journal
/
Neurociência
/
In vivo Fødselsdepression Elektroporation og Time-lapse Imaging af neuroblast Migration i Mouse Akut hjerneskiver
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In vivo Fødselsdepression Elektroporation og Time-lapse Imaging af neuroblast Migration i Mouse Akut hjerneskiver

DOI:
10.3791/50905-v

•

10:51 min

•

November 25, 2013

•

, , , ,
1Wolfson Centre for Age-Related Diseases,King’s College London, 2David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology

Capítulos

  • 00:05Título
  • 01:30Postnatal Electroporation
  • 03:36Preparation of Acute Brain Slice Cultures
  • 05:41Time-lapse Imaging of Neuroblast Migration
  • 06:57Analyzing Neuroblast Migration
  • 08:51Results: Time-lapse Imaging of Migrating Neuroblasts in Mouse Acute Brain Slices
  • 10:13Conclusion

Summary

Tadução automática

Tadução automática

Neuroblast migration er en grundlæggende begivenhed i postnatal neurogenesis. Vi beskriver en protokol til effektiv mærkning af neuroblasts ved in vivo postnatal elektroporation og efterfølgende visualisering af deres migration ved hjælp af time-lapse billeddannelse af akutte hjernen skiver. Vi inkluderer en beskrivelse af den kvantitative analyse af neuroblast dynamik ved video tracking.

Tags

Keywords: Subventricular ZoneNeurogenic NicheNeuroblast MigrationRostral Migratory StreamOlfactory BulbIn Vivo ElectroporationTime-lapse ImagingAcute Brain SlicesNeurogenesisBrain Repair

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/50905?article_type=v

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain
check_url/pt/50905?article_type=v

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain
check_url/pt/50905?article_type=v

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
check_url/pt/50905?article_type=v

Funktionel Neuroimaging Med Ultrasonic blod-hjerne barrieren forstyrrelser og mangan-forstærket MRI
check_url/pt/50905?article_type=v

Time-lapse Imaging af neuroblast Migration i Akutte Skiver af den voksne Mouse forhjerne
check_url/pt/50905?article_type=v

Dobbelte Elektrofysiologiske Optagelser af synaptisk-evoked astrogliale og Neuronal respons i Akut hippocampusskiver
check_url/pt/50905?article_type=v

Langsigtet Time Lapse Imaging af Mouse Cochlear Eksplantater
check_url/pt/50905?article_type=v

Ex Vivo Imaging af Postnatal cerebellare Granule Cell Migration Brug Konfokal makroskopi
check_url/pt/50905?article_type=v

Time-lapse konfokal billeddannelse af migrerende neuroner i organotypisk skive kultur af embryonale mus hjernen ved hjælp af<em> I Utero</em> Elektroporation
check_url/pt/50905?article_type=v

To-foton billeddannelse af Microglial processer tiltrækning mod ATP eller Serotonin i akut hjernen skiver

Read Article