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JoVE Journal Developmental Biology
Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
 

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

Article DOI: 10.3791/52049-v • 11:22 min • January 12th, 2015
January 12th, 2015 •
1,2, 1, 1, 1, 1
1Centre of Human and Aerospace Physiological Sciences, King's College London, 2Wellcome Trust-Medical Research Council, Cambridge Stem Cell Institute

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Resumo
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The main adherent cell types derived from human muscle are myogenic cells and fibroblasts. Here, cell populations are enriched using magnetic-activated cell sorting based on the CD56 antigen. Subsequent immunolabelling with specific antibodies and use of image analysis techniques allows quantification of cytoplasmic and nuclear characteristics in individual cells.

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Keywords: Skeletal Muscle Satellite Cells Myogenic Cells Muscle Precursor Cells Fibroblasts CD56 Desmin TE-7 Enzymatic Digestion Collagenase Dispase Magnetic Activated Cell Sorting (MACS) Immunocytochemistry Cell Culture Muscle Regeneration
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