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JoVE Journal Developmental Biology
Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure
 

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Article DOI: 10.3791/59580-v • 08:00 min • August 23rd, 2019
August 23rd, 2019 •
*1, *1, 2, 1, 2, 1
1Division of Nutritional Sciences, Cornell University, 2Meinig School of Biomedical Engineering, Cornell University
* These authors contributed equally

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Resumo
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We present techniques for isolating, culturing, characterizing, and differentiating human primary muscle progenitor cells (hMPCs) obtained from skeletal muscle biopsy tissue. hMPCs obtained and characterized through these methods can be used to subsequently address research questions related to human myogenesis and skeletal muscle regeneration.

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Isolation Culture Characterization Differentiation Human Muscle Progenitor Cells Skeletal Muscle Biopsy Regenerative Process Donor Variability Phenotypic Analysis Proliferation Differentiation Capabilities Yield Purity Reproducibility Visualization Sterile Conditions Petri Dish Scalpel Mince Tissue 15-milliliter Tube PBS
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