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Isolatie en Kwantitatieve Immunocytochemische karakterisering van primaire Myogene cellen en fibroblasten van Human Skeletal Muscle
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
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Isolatie en Kwantitatieve Immunocytochemische karakterisering van primaire Myogene cellen en fibroblasten van Human Skeletal Muscle

15,618 Views

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11:22 min

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January 12, 2015

DOI:

10.3791/52049-v

DOI:
10.3791/52049-v

•

11:22 min
January 12, 2015

•

4 Views
, , , ,
1Centre of Human and Aerospace Physiological Sciences,King’s College London, 2Wellcome Trust-Medical Research Council,Cambridge Stem Cell Institute

Tags

Skeletal MuscleSatellite CellsMyogenic CellsMuscle Precursor CellsFibroblastsCD56DesminTE-7Enzymatic DigestionCollagenaseDispaseMagnetic Activated Cell Sorting (MACS)ImmunocytochemistryCell CultureMuscle Regeneration

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Agley, C. C., Rowlerson, A. M., Velloso, C. P., Lazarus, N. L., Harridge, S. D. R. Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle. J. Vis. Exp. (95), e52049, doi:10.3791/52049 (2015).

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