Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish

Yali Zhao; Nancy L. Wayne

doi:10.3791/50312

Journal

/

Neurociência

/

鉴定转基因鱼的完整的大脑中的神经元的电活动记录

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Neurociência

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JoVE Journal Neurociência

Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish

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鉴定转基因鱼的完整的大脑中的神经元的电活动记录

DOI:

10.3791/50312-v

•

07:45 min

•

April 30, 2013

•

Yali Zhao, Nancy L. Wayne

¹Department of Physiology,University of California, Los Angeles

Capítulos

00:05Título
01:27Dissection of Brains from Adult Medaka
02:56Mounting the Brain in a Recording Chamber
03:37Electrophysiology: Loose Patch Recording of Action Potential Firing
05:36Electrophysiology: Whole Cell Recording of Membrane Potential
06:26GnRH3 Neuron Activity in Medaka and Zebrafish
07:27Conclusion

Summary

Tadução automática

在这段视频中，我们将演示如何记录保留复杂的神经回路在整个大脑的准备，从确定单个神经元的电活动。我们使用转基因鱼中，促性腺激素释放激素（GnRH）的神经元与用于识别完整的大脑制备一种荧光蛋白基因的标签。

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