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ハイブリダイゼーション<em>その場で</em>唾液腺、卵巣、および媒介蚊の胚の
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
Please note that all translations are automatically generated. Click here for the English version.

ハイブリダイゼーション<em>その場で</em>唾液腺、卵巣、および媒介蚊の胚の

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10:58 min

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June 28, 2012

DOI:

10.3791/3709-v

DOI:
10.3791/3709-v

•

10:58 min
June 28, 2012

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10 Views
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1Department of Molecular Biology and Biochemistry,University of California, Irvine, 2Department of Microbiology and Molecular Genetics,University of California, Irvine

Summary

Automatically generated

時間的·空間的遺伝子発現解析は、ゲノム機能解析において重要な役割を持っています。ホールマウントハイブリダイゼーション<em>その場で</em>組織や細胞内コンパートメント内での転写産物の局在を決定するために有用である。ここでは、ハイブリダイゼーションを概説<em>その場で</em蚊の特定の標的組織のために修正を加えた>プロトコル。

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Hybridization In SituSalivary GlandsOvariesEmbryosVector MosquitoesPathogensTranscriptsGene RegulatorsMosquito-borne Disease TransmissionEgg DevelopmentGene Expression RegulationSpatial InformationAccumulation PatternsFemale Salivary GlandsOvaries And Embryos Subcellular CompartmentsMosquito Reproduction And DevelopmentRNA Probe PreparationFixation And Hybridization

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Cite this Article

Juhn, J., James, A. A. Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes. J. Vis. Exp. (64), e3709, doi:10.3791/3709 (2012).

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