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宣传和检测Maedi visna病毒传染性表达绿色荧光蛋白的分子克隆
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein
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宣传和检测Maedi visna病毒传染性表达绿色荧光蛋白的分子克隆

DOI:
10.3791/3483-v

•

08:49 min

•

October 09, 2011

•

,
1Institute for Experimental Pathology,University of Iceland

Capítulos

  • 00:05Título
  • 01:19Transfection
  • 05:13Titration of Virus
  • 07:13FACS Analysis of Infected Cells

Summary

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我们描述了一个maedi visna病毒的分子克隆，表达GFP和充分传染病。这种病毒的复制，可以通过使用荧光显微镜和流式细胞仪检测。

Tags

Maedi-visna VirusLentivirusInterstitial PneumoniaEncephalitisTarget CellsMonocyte LineageCell TypesGreen Fluorescent ProteinEgfp GeneDUTPase GeneReplicationCAEVMolecular CloneIn VitroIn VivoPrimary Cell LineChoroid PlexusTransfectionPropagationInfectiousEGFP ExpressionPassagesTCID50Infected CellsCell TropismPathogenicity

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