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Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein
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Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein
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October 09, 2011
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Summary
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We describe a molecular clone of maedi-visna virus that expresses GFP and is fully infectious. Replication of this virus can be detected by using fluorescence microscopy and flow cytometry.
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Maedi-visna VirusLentivirusInterstitial PneumoniaEncephalitisTarget CellsMonocyte LineageCell TypesGreen Fluorescent ProteinEgfp GeneDUTPase GeneReplicationCAEVMolecular CloneIn VitroIn VivoPrimary Cell LineChoroid PlexusTransfectionPropagationInfectiousEGFP ExpressionPassagesTCID50Infected CellsCell TropismPathogenicity
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Jónsson, S. R., Andrésdóttir, V. Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein. J. Vis. Exp. (56), e3483, doi:10.3791/3483 (2011).
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