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伝播と緑色蛍光タンパク質を発現Maedi -ビスナウイルスの感染分子クローンの検出
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October 09, 2011
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Summary
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我々はGFPを発現し、完全に伝染性であるmaedi -ビスナウイルスの分子クローンを説明します。このウイルスの複製は、蛍光顕微鏡やフローサイトメトリーを用いて検出することができます。
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Maedi-visna VirusLentivirusInterstitial PneumoniaEncephalitisTarget CellsMonocyte LineageCell TypesGreen Fluorescent ProteinEgfp GeneDUTPase GeneReplicationCAEVMolecular CloneIn VitroIn VivoPrimary Cell LineChoroid PlexusTransfectionPropagationInfectiousEGFP ExpressionPassagesTCID50Infected CellsCell TropismPathogenicity
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Jónsson, S. R., Andrésdóttir, V. Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein. J. Vis. Exp. (56), e3483, doi:10.3791/3483 (2011).
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