Single Cell Fate Mapping in Zebrafish

Vikram Kohli; Kira Rehn; Saulius Sumanas

doi:10.3791/3172

Journal

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Biologia

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Zebra balığı Tek Hücre Kader Haritalama

JoVE Journal
Biologia

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JoVE Journal Biologia

Single Cell Fate Mapping in Zebrafish

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Zebra balığı Tek Hücre Kader Haritalama

13,202 Views

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07:53 min

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October 05, 2011

DOI:

10.3791/3172-v

DOI:

10.3791/3172-v

•

07:53 min

October 05, 2011

•

3 Views

Vikram Kohli¹, Kira Rehn¹, Saulius Sumanas^1,2

¹Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, ²Division of Hematology/Oncology,Cincinnati Children’s Hospital Medical Center

Summary

Safir femtosaniye lazer osilatör: Ti bir yöntem olup, bir iki-fotonlu emme ile bir kafes floresan proteini içeren tek bir hücre photoactivate için tarif edilmiştir. Kader haritası için fotoaktive hücre, immunohistokimya kullanılır. Bu teknik, her hücre tipi için de uygulanabilir.

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Single Cell Fate Mapping Zebrafish Biologia do Desenvolvimento Fate Mapping Lineage Tracing Undifferentiated Precursor Cells Photoactivatable Proteins Fluorescence Spectrum UV Excitation Cell Tracing Probes Long-term Cell Tracking Caged Fluorescein-dextran Embryo Model Systems Uncaging Fluorescein-dextran Spatial Resolution Photoactivation Near-infrared Laser Pulses Titanium Sapphire Femtosecond Laser Two-photon Confocal Microscopes

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Zebra balığı Tek Hücre Kader Haritalama

Summary

Automatically generated

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