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Competitive Genomic Screens of Barcoded Yeast Libraries
JoVE Journal
Biologia
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JoVE Journal Biologia
Competitive Genomic Screens of Barcoded Yeast Libraries
DOI:

11:59 min

August 11, 2011

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Capítulos

  • 00:05Título
  • 01:55Combining Individual Mutants into a Single Pool
  • 03:23Experimental Pool Growth
  • 04:56Genomic DNA Extraction, PCR and Microarray Hybridization
  • 08:33Sequencing Barcoded Yeast Strains
  • 09:22Genomic Screening Results
  • 11:03Conclusion

Summary

Tadução automática

We have developed comprehensive, unbiased genome-wide screens to understand gene-drug and gene-environment interactions. Methods for screening these mutant collections are presented.

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