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Genetics
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JoVE Journal Genetics

使用腺相关病毒（AAV）和 2 细胞胚胎电穿孔对大鼠胚胎进行 CRISPR-Cas9 基因组编辑

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使用腺相关病毒（AAV）和 2 细胞胚胎电穿孔对大鼠胚胎进行 CRISPR-Cas9 基因组编辑

Article DOI: 10.3791/66069-v • 08:24 min • March 15th, 2024

March 15th, 2024 •

Daniel J. Davis^1,2,4, James F. McNew², Jennifer N. Walls³, Christine E. Bethune³, Payton S. Oswalt³, Elizabeth C. Bryda^1,2,3,4

¹Animal Modeling Core, University of Missouri, ²Comparative Medicine Program, University of Missouri, ³Rat Resource and Research Center, University of Missouri, ⁴Department of Veterinary Pathobiology, University of Missouri

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该协议提出了一种用于生产转基因大鼠模型的优化方法。腺相关病毒（AAV）用于递送 DNA 修复模板，电穿孔用于递送 CRISPR-Cas9 试剂以完成 2 细胞胚胎的基因组编辑过程。

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本月在JoVE上，第205期，腺相关病毒（AAV），基因组编辑，CRISPR-Cas，电穿孔，2细胞胚胎，敲入，大鼠动物模型

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