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Journal / Genetics / CRISPR-Cas9 Genome Editing of Rat Embryos using…
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JoVE Journal Genetics
CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation
 

CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation

Article DOI: 10.3791/66069-v • 08:24 min • March 15th, 2024
March 15th, 2024 •
1,2,4, 2, 3, 3, 3, 1,2,3,4
1Animal Modeling Core, University of Missouri, 2Comparative Medicine Program, University of Missouri, 3Rat Resource and Research Center, University of Missouri, 4Department of Veterinary Pathobiology, University of Missouri

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Summary
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This protocol presents an optimized approach for producing genetically modified rat models. Adeno-associated virus (AAV) is used to deliver a DNA repair template, and electroporation is used to deliver CRISPR-Cas9 reagents to complete the genome editing process in 2-cell embryo.

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Adeno-associated virus (AAV) genome editing CRISPR-Cas electroporation 2-cell embryos knock-in rat animal model
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