Journal
/
/
CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation
JoVE Journal
Genetics
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation

CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation

1,144 Views

08:24 min

March 15, 2024

DOI:

08:24 min
March 15, 2024

23 Views
, , , , ,

Summary

Automatically generated

This protocol presents an optimized approach for producing genetically modified rat models. Adeno-associated virus (AAV) is used to deliver a DNA repair template, and electroporation is used to deliver CRISPR-Cas9 reagents to complete the genome editing process in 2-cell embryo.

Read Article