This protocol presents an optimized approach for producing genetically modified rat models. Adeno-associated virus (AAV) is used to deliver a DNA repair template, and electroporation is used to deliver CRISPR-Cas9 reagents to complete the genome editing process in 2-cell embryo.
J. Davis, D., McNew, J. F., Walls, J. N., Bethune, C. E., Oswalt, P. S., Bryda, E. C. CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation. J. Vis. Exp. (205), e66069, doi:10.3791/66069 (2024).